Journal: Journal of Neuroscience Research
Article Title: The Functional Epididymal Amyloid Cystatin‐Related Epididymal Spermatogenic ( CRES ) is a Component of the Mammalian Brain Extracellular Matrix
doi: 10.1002/jnr.70113
Figure Lengend Snippet: CRES in present in the loose/interstitial extracellular matrix. (A) Immunofluorescence analysis showed CRES (magenta fluorescence) colocalized with the ECM marker Wisteria Floribunda Agglutinin (WFA, green fluorescence) in the female and male cortical ECM from CD‐1 mice. (magenta+ green = lilac/white). Scale bar, 20 μm, 10 μm. (B) CRES (green fluorescence) colocalized with the ECM marker phosphacan (magenta fluorescence) in the hippocampal ECM from CD‐1 mice. (green + magenta = lilac/white). Scale bar, 20 μm, 10 μm. Blue, DAPI staining of nuclei. Data are representative of n = 3 female and n = 3 male mice.
Article Snippet: To determine cell type‐specific expression of CRES, we co‐stained the tissues with NeuN (ab104224 RRID:AB_10711040, mouse monoclonal, 3 μg/mL, Abcam Waltham, MA) (Gusel'nikova and Korzhevskiy ), a marker for neurons; Glial Fibrillary Acidic Protein (GFAP, ab4674 RRID:AB_304558, chicken polyclonal 1:1000, Abcam, Waltham MA) (Zhang et al. ), a marker for astrocytes; or Phosphacan (clone 3F8 mouse anti‐rat, 5 μg/mL, Developmental Studies Hybridoma Bank Iowa City, IA) (Garwood et al. ), a marker for ECM for 2 h at room temperature.
Techniques: Immunofluorescence, Fluorescence, Marker, Staining